ELISA/assay

Soluble Leptin Receptor (sLEP-R) ELISA Assay Kit

R07

1 x 96 well

690 USD

Soluble Leptin Receptor (sLEP-R) ELISA Assay Kit

This ELISA for Soluble Leptin Receptor (sLEP-R) is a so-called Sandwich-Assay using two specific and highly affine antibodies. The sLEP-R in the samples binds to the first antibody coated on the microtiter plate. In the following step the second specific anti-sLEP-R-antibody binds in turn to the immobilised sLEP-R. This is biotinylated and allows the binding of a streptavidin-peroxidase enzyme conjugate. Subsequently, the peroxidase catalyzes an enzymatic reaction resulting in a blue coloration. The intensity of the blue color depends on the sLEP-R content of the sample. The reaction is stopped by the addition of stop solution. After stopping the reaction, the color intensity (then yellow) is quantified by measuring the absorbance and converted to the sLEP-R concentration using a standard curve.

ELISA

Assay Kit

Endocrinology

1 x 96 well

Leptin

Leptin

0.0385 ng/mL

30 uL

Serum or Plasma

5 hours

Human

RUO

wet

2-8C

690 USD