Prostatic Specific Antigen – Total (PSA) CLIA | Eagle BioSciences PSA31-L01 product information

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product summary

company name :

Eagle BioSciences

product type :

ELISA/assay

product name :

Prostatic Specific Antigen – Total (PSA) CLIA

catalog :

PSA31-L01

quantity :

1 x 96 well

product information

Eagle Catalog Number :

PSA31-L01

Description :

Prostatic Specific Antigen – Total (PSA) CLIA

Assay Principle :

The principle of this chemiluminescence immunoassay (LIA) test follows a typical one-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for total PSA is immobilized onto the microplate and another monoclonal antibody specific for a different region of PSA is conjugated to horse radish peroxidase (HRP). Total PSA from the sample and standards are allowed to bind to the plate, washed, and subsequently incubated with the HRP conjugate. After a second washing step, the luminescence substrate is added and the relative luminescence units (RLUs) are measured in a microplate luminometer. The RLUs formed by the enzymatic reaction are directly proportional to the concentration of total PSA in the samples. A set of standards is used to plot a standard curve from which the concentrations of tPSA in patient and control samples are read.

Product Type :

CLIA

Product Category :

Assay Kit

Product Subcategory :

Steroid

Size :

1 x 96 well

Biomarker :

Prostatic

Keywords :

Prostatic

Sensitivity :

0.082 ng/mL

Standard Range :

0.1–100 ng/mL

Sample Size :

50 μL

Sample Type :

Serum

Incubation Time :

120 minutes

Species :

Human

Regulatory Status :

RUO

Shipping :

wet

Storage :

2-8C