ELISA/assay

Progesterone Saliva Sensitive ELISA

PRO32-K01

1 x 96 well

500 USD

Progesterone Saliva Sensitive ELISA

The Salivary Progesterone EIA kit is based on the competition principal and microplate separation. Progesterone calibrators and unknown amounts of progesterone in saliva samples compete with a fixed amount of progesterone conjugated to horse radish peroxidase (Progesterone-HRP) for binding sites with a rabbit progesterone monoclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away, enzyme substrate solution is added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm. The amount of Progesterone-HRP detected is inversely proportional to the amount of progesterone in a sample.

ELISA

Assay Kits

Steroid

1 x 96 well

Progesterone

Progesterone

1.477 pg/ml

10 - 1000 pg/mL

50 µl

Saliva

2 hours

Human

RUO

wet

2-8C

500 USD