ELISA/assay

Horse Serum Amyloid A (SAA) ELISA

OSA91-K01

1 x 96 well

760 USD

Horse Serum Amyloid A (SAA) ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-SAA, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay Kit

Veterinary

1 x 96 well

Serum Amyloid A (SAA) ELISA

Serum Amyloid A (SAA) ELISA

0.962 ng/ml

2.25 ng/ml - 72 ng/ml

100 µl

Serum, plasma

110 minutes

Horse

RUO

wet

2-8C

760 USD