ELISA/assay

Mouse Cystatin C ELISA

OCC11-K01

1 x 96 well

665 USD

Mouse Cystatin C ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Cystatin C present in samples reacts with the anti-CYS antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-CYS antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CYS. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CYS in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CYS in the test sample. The quantity of CYS in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay Kit

Veterinary

1 x 96 well

Cystatin C

Cystatin C

0.146 ng/ml

1.25 ng-ml - 40 ng/ml

100 µl

Serum, plasma, urine

100 minutes

Mouse

RUO

wet

2-8C

665 USD