ELISA/assay

Hamster (CHO) Nidogen-1 ELISA Assay Kit

NDG91-K01

96 wells

1510 USD

Hamster (CHO) Nidogen-1 ELISA Assay Kit

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Nidogen-1 (NDN) present in samples reacts with the anti-NDN antibodies which have been absorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-NDN is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added, and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of NDN in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of NDN in the test sample. The quantity of NDN in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.

ELISA

ASSAY kit

Immunology

96 wells

Nidogen-1

Nidogen-1

0.3125 ng/mL - 20 ng/mL

100 μL

Tissue Culture Fluids

2 hours 50 minutes

CHO, Hamster

RUO

wet

2-8°C

1510 USD