ELISA/assay

Myeloperoxidase (MPO) Stool, Urine ELISA

MPO39-K01

1 x 96 well

710 USD

Myeloperoxidase (MPO) Stool, Urine ELISA

Assay standards, controls and extracted patient samples are added directly to wells of a microtiter plate that is coated with antibody to myeloperoxidase. After an incubation period, the plate is washed and horseradish peroxidase (HRP) conjugated human myeloperoxidase antibody is added to each well. After the second incubation period, a “sandwich” of solid-phase monoclonal antibody - human myeloperoxidase – HRP conjugated antibody” is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and the absorbances are then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human myeloperoxidase in the test sample. A standard curve is generated by plotting the absorbance versus the respective human myeloperoxidase concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of human myeloperoxidase in test samples is determined directly from this standard curve.

ELISA

Assay Kits

Gastroenterology

1 x 96 well

MPO

MPO, Myeloperoxidase

0.3 ng/ml

2 - 512 ng/ml

50 mg/100ml

Stool, Urine

2.5 hours

Human

RUO

wet

2-8C

710 USD