ELISA/assay

Lactoferrin ELISA

LFN35-K01

1 x 96 wells

925 USD

Lactoferrin ELISA

The human lactoferrin ELISA kit is a solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured human lactoferrin. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human lactoferrin standards (log). The human lactoferrin concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

ELISA

Assay Kit

Cardiovascular

1 x 96 wells

Lactoferrin

Lactoferrin

0.4 ng/mL

0.4 - 100 ng/mL

100 uL

Plasma, Urine, Feces, Breast Milk, Cell Culture Supernatant

4 hours 30 minutes

Human

RUO

wet

2-8C

925 USD