ELISA/assay

Human IL-17 ELISA KIT

L1731-K01

1 x 96 well

530 USD

Human IL-17 ELISA KIT

The Eagle Biosciences Human Interleukin 17 (IL-17) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 17 (IL-17) concentrations in cell culture supernates, serum, and plasma. The Eagle Biosciences Human Interleukin 17 (IL-17) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

The Eagle Biosciences Human Interleukin 17 (IL-17) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-17 is added to the wells and binds to the combination of capture antibody-IL-17 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-17 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-17 standard dilutions and IL-17 sample concentration determined.

ELISA

Assay Kits

Oxidative Stress

1 x 96 well

il-17

Interleukin 17, IL-17

7 pg/ml

15.65 - 500 pg/ml

100 µl

Serum, Plasma, Cell Culture

3.5 hours

Human

RUO

wet

2-8C

530 USD