ELISA/assay

COVID-19 Neutralizing Antibody Quantitatinve ELISA Assay Kit

KTR-1036

1 x 96 well

865 USD

COVID-19 Neutralizing Antibody Quantitatinve ELISA Assay Kit

This ELISA kit is designed, developed, and produced for the quantitative measurement of the neutralizing antibodies to SARS-CoV-2 RBD of its spike protein in human serum This assay utilizes the microplate-based enzyme immunoassay technique. Assay calibrators, controls, and human serum samples are added to the microtiter wells of a microplate coated with streptavidin. Simultaneously, horseradish peroxidase (HRP) labeled COVID-19 recombinant spike protein and biotinylated angiotensin converting enzyme-2 (ACE-2) are added to each well. After the first incubation period, the unbound protein matrix is removed with a subsequent washing step; a complex of "Streptavidin---Biotin-ACE2---HRP-COVID-19 recombinant spike protein" is formed. If there is specific COVID-19 neutralizing antibody present in the tested specimen, the formation of the above complex is blocked. A color reaction with a substrate solution in a timed reaction is measured in a spectrophotometric microplate reader. The HRP enzymatic activity of the complex on the wall of the microtiter well is inversely proportional to the amount of the COVID-19 neutralizing antibody level in the tested specimen.

ELISA

Assay Kits

SARS-CoV-2

1 x 96 well

COVID-19 Neutralizing Antibody

COVID-19 Neutralizing Antibody

0 U/ml - 100 U/ml

50 µl

serum

1 hr 5 mins

Human

RUO

wet

2-8C

865 USD