ELISA/assay

Nitrotyrosin (EDTA-Plasma) ELISA Assay Kit

KR7829

1 x 96 well

1250 USD

Nitrotyrosin (EDTA-Plasma) ELISA Assay Kit

This ELISA is designed for the quantitative determination of nitrotyrosine. The assay utilises the “sandwich” technique. Standards, controls and prepared samples which are assayed for nitrotyrosine are added into the wells of a micro plate coated with polyclonal goat anti- nitrotyrosine antibody. During the first incubation step, nitrated proteins are bound by the immo-bilised primary antibody. Then a peroxidase-conjugated polyclonal goat anti-human serum proteins antibody is added into each microtiter well and a “sandwich” of primary antibody - nitrated protein – peroxidase-conjugate is formed. Tetramethylbenzidine is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The colour changes from blue to yellow. The intensity of the yellow colour is directly proportional to the concentration of nitrotyrosine. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. standard concentration is generated, using the values obtained from the standards.

ELISA

Assay Kits

Cardiovascular

1 x 96 well

Nitrotyrosine

4.9-400 nM

15 µl (EDTA Plasma, Serum) 50 µl (dried blood)

EDTA Plasma, Serum, Dried Blood Spots

2.25 hours

Human

RUO

wet

2-8C

1250 USD