ELISA/assay

L-Kynurenic Acid (Urine) ELISA Assay Kit

KR7735

1 x 96 well

1210 USD

L-Kynurenic Acid (Urine) ELISA Assay Kit

This ELISA is designed for the quantitative determination of kynurenic acid. The assay is based on the method of competitive enzyme linked immunoassays. Samples, standards and controls are incubated in wells of a microtiter plate coated with kynurenic acid (antigen), together with a peroxidase labeled polyclonal anti-kynurenic acid antibody. During the incubation period the free target antigen in the sample competes with the antigen immobilized on the wall of the microtiter wells for the binding of the peroxidase-labelled polyclonal antibodies. After a washing step to removve the unbound components, the peroxidase substrate tetramethylbenzidine (TMB) is added. Finally, the enzymatic reaction is terminated by an acidic stop solution. The colour changes from blue to yellow and the absorbance is measured in the photometer at 450 nm. The intensity of the yellow colour is inverse proportional to the kynurenic acid concentration in the sample; this means, high antigen concentration in the sample reduces the concentration of antibodies bound to the antigen on the plate and lowers the photometric signal. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standards. Kynurenic acid, present in the patient samples, is determined directly from this curve.

ELISA

Assay Kits

Oxidative Stress

1 x 96 well

Kynurenic Acid (Urine) ELISA

1.5-150 µM

50 µL

Urine

Overnight

Human

RUO

wet

2-8C

1210 USD