ELISA/assay

Lysozyme (Stool) ELISA Assay Kit

KR6900

1 x 96 well

740 USD

Lysozyme (Stool) ELISA Assay Kit

The assay utilizes the “sandwich” technique with two selected antibodies that recognise human lysozyme. Standards, controls and diluted samples, which are assayed for human lysozyme, are added into the wells of a micro plate coated with a high affine anti-human lysozyme antibody. During the first incubation step, lysozyme is bound by the immobilised antibody. Then a peroxidase-conjugated anti-human lysozyme antibody is added into each microtiter well and a “sandwich” of capture antibody - human lysozyme – peroxidase-conjugate is formed. Tetramethylbenzidine is used as peroxidase sub-strate. Finally, an acidic stop solution is added to terminate the enzymatic reaction. The colour changes from blue to yellow. The intensity of the yellow colour is directly proportional to the concentration of lysozyme. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards. Lysozyme present in the samples is determined directly from this curve.

ELISA

Assay Kits

Immunology

1 x 96 well

Stool) ELISA

1.1-30 ng/ml

15 mg

Stool

2.25 hours

Human

RUO

wet

2-8C

740 USD