ELISA/assay

PMN-Elastase (Serum/Plasma) ELISA Assay Kit

KR6841

1 x 96 well

745 USD

PMN-Elastase (Serum/Plasma) ELISA Assay Kit

In this Eagle Biosciences Assay, PMN elastase in the sample is bound to polyclonal rabbit- anti-PMN elastase antibodies (in excess), which are immobilised on the surface of the microtiter wells (PLATE). To remove all unbound substances, a washing step is carried out. In a second incubation step, a monoclonal mouse-anti-PMN elastase antibody (AB) is added. This antibody is able to detect both the free and the complexed form with the specific inhibitor (α1-proteinase inhibitor = α1-antitrypsin). The quantification of the bound PMN elastase is carried out by adding an anti-mouse peroxidase- labeled conjugate (CONJ). Finally, the PMN elastase – antigen – antibody complex is incubated with the peroxidase substrate, tetramethylbenzidine (SUB). An acidic stop solution (STOP) is then added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of PMN elastase in the sample. A dose response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using the values obtained from the calibrators. PMN elastase, present in the patient samples, is determined directly from this curve. The combination of two specific antibodies in the PMN elastase ELISA drastically reduces the possibility of false results and offers a reliable diagnostic system to the user.

ELISA

Assay Kits

Gastrointestinal

1 x 96 well

Pancreatic Elastase

Pancreatic Elastase

0.011ng/ml

0.37-10 ng/ml

20 µl

Serum, plasma

1.25 hours

Human

RUO

wet

2-8C

745 USD