ELISA/assay

MutaPLATE® Lactase (TM) TaqMan (32)

KF1907232

32 tests

1105 USD

MutaPLATE® Lactase (TM) TaqMan (32)

The Eagle Biosciences MutaPLATE® Laktase (TAQ-Man) real time PCR Kit contains specific primers and additional material for the detection of the T/C (-13910) polymorphism in the regulatory region of the lactase phlorizin hydrolase gene. The variable area of the regulatory region from lactase gene is amplified by PCR using genomic DNA-template. The specific primers used in the kit flank the variable area of lactase gene (LCT) and generate an amplificate of 300 bp using Taq-Man technology. The standard PCR contains additionally two sequence specific oligonucleotides marked with fluorescence dye (TaqMan probes). Both probes bind at the amplificated target-DNA which includes the single nucleotide polymorphism (SNP). Due to this, a fluorescence signal is generated and detected by the optical unit of the used real time PCR instrument. The TaqMan probe for the C-allele (mutation) is marked with FAM (510nm, green) and the TaqMan probe for the T-allele (wildtype) is marked with YAK (555 nm, yellow).

Molecular Assay

Assay Kits

Molecular

32 tests

Lactase

MutaPLATE® Lactase (TM) TaqMan (32)

200 µl

DNA (e.g.whole blood, cheek swab)

RUO

Dry Ice

-20C

1105 USD