Trastuzumab (HERCEPTIN) ELISA | Eagle BioSciences KBI1017 product information

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product summary

company name :

Eagle BioSciences

product type :

ELISA/assay

product name :

Trastuzumab (HERCEPTIN) ELISA

catalog :

KBI1017

quantity :

1 x 96 well

product information

Eagle Catalog Number :

KBI1017

Description :

Trastuzumab (HERCEPTIN) ELISA

Assay Background :

Trastuzumab is a humanized monoclonal antibody which targets Her-2/neu receptor. Trastuzumab is approved for use in treating breast cancers and is being tested for other cancers that overexpress HER-2/neu.

Assay Principle :

The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Trastuzumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Trastuzumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Trastuzumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Trastuzumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Product Type :

ELISA

Product Category :

Assay Kits

Product Subcategory :

Therapeutic Drug Monitoring

Size :

1 x 96 well

Biomarker :

Trastuzumab

Keywords :

Trastuzumab

Sensitivity :

<2.5 ng/mL

Standard Range :

2.5-160 ng/ml

Sample Size :

100 uL

Sample Type :

Serum, Plasma, Cell Culture Supernatants

Incubation Time :

2.5 hours

Regulatory Status :

RUO

Shipping :

wet

Storage :

2-8C