ELISA/assay

N8-Acetyl-Spermidine ELISA Assay kit

IS-I-2500R

1 x 96 wells

1245 USD

N8-Acetyl-Spermidine ELISA Assay kit

Enzyme Immunoassay for the quantitative determination of N8-Acetyl-Spermidine (N8-actSp) in plasma samples. After precipitation and derivatization N8-Acetyl-Spermidine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.

ELISA

Assay Kit

Cardiovascular

1 x 96 wells

N8-Acetyl-Spermidine, N8-AcSpdn

N8-Acetyl-Spermidine, N8-AcSpdn

0.5 nM

2.4 - 93.75 nM

20 uL

Plasma, Cell culture supernatant

Overnight

All Species

RUO

wet

2-8C

1245 USD