ELISA/assay

L-Histidine ELISA Assay kit

IS-I-1300R

96 wells

975 USD

L-Histidine ELISA Assay kit

Enzyme Immunoassay for the quantitative determination of L-Histidine (His) in plasma samples. After extraction and derivatization L-Histidine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.

ELISA

ASSAY kit

Cardiovascular

96 wells

Histidine

Histidine

1.4 uM (0.3 ug/mL)

5.3-390µM (1.1 – 81.8µg/mL)

10 uL

Plasma

Overnight

RUO

wet

2-8°C

975 USD