ELISA/assay

Human IL-8 ELISA KIT

IL831-K01

1 x 96 well

530 USD

Human IL-8 ELISA KIT

The Eagle Biosciences Human Interleukin 8 (IL-8) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 8 (IL-8) concentrations in cell culture supernates, serum, and plasma. The Eagle Biosciences Human Interleukin 8 (IL-8) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-8 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-8 is added to the wells and binds to the combination of capture antibody-IL-8 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-8 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-8 standard dilutions and IL-8 sample concentration determined.

ELISA

Assay Kits

Oxidative Stress

1 x 96 well

IL-8

Interleukin 8, IL-8

4 pg/ml

31.25 - 1000 pg/ml

100 µl

Serum, Plasma, Cell Culture

3.5 hours

Human

RUO

wet

2-8C

530 USD