ELISA/assay

Dog sIL2-R ELISA Kit

IL241-K01

96 wells

880 USD

Dog sIL2-R ELISA Kit

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Soluble Interleukin-2 Receptor (SLR) present in samples reacts with the anti-SLR antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-SLR, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SLR in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SLR in the test sample. The quantity of SLR in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay Kit

Immunology

96 wells

sIL2-R

sIL2-R

0.08 - 5 ng/mL

100 uL

Plasma, Serum, Tissue Culture Fluids

1 hour 50 minutes

Canine

RUO

wet

2-8C

880 USD