ELISA/assay

Human IFN-γ ELISA KIT

IFG31-K01

1 x 96 well

530 USD

Human IFN-γ ELISA KIT

The Eagle Biosciences Human Interferon Gamma (IFN-γ) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interferon Gamma (IFN-γ) concentrations in cell culture supernates, serum, and plasma. The Eagle Biosciences Human Interferon Gamma (IFN-γ) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

The Eagle Biosciences Interferon Gamma (IFN-γ) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IFN-γ is added to the wells and binds to the combination of capture antibody- IFN-γ in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IFN-γ present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IFN-γ standard dilutions and IFN-γ sample concentration determined.

ELISA

Assay Kits

Immunology

1 x 96 well

IFN-Gamma

Interferon Gamma, IFN-γ

7 pg/ml

15.625-1000 pg/ml

100 µl

Serum, Plasma, Cell Culture

3.5 hours

Human

RUO

wet

2-8C

530 USD