ELISA/assay

Human Albumin ELISA

HUA39-K01

1 x 96 well

435 USD

Human Albumin ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Albumin present in samples reacts with the anti-Albumin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Alb antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Alb. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alb in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alb in the test sample. The quantity of Alb in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay kit

Endocrine

1 x 96 well

Albumin

Albumin

1.900 ng/ml

6.25-200 ng/ml

100 µl

Biological Fluids

100 minutes

Human

RUO

wet

2-8C

435 USD