ELISA/assay

Human Serum Amyloid A (SAA) ELISA

HSA31-K01

1 x 96 well

630 USD

Human Serum Amyloid A (SAA) ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed f rom the standards, and corrected for sample dilution.

ELISA

Assay Kit

Immunology

1 x 96 well

Serum Amyloid A (SAA) ELISA

Serum Amyloid A (SAA) ELISA

0.492 ng/ml

1.56 ng/ml - 100 ng/ml

100 µl

Serum, plasma

90 minutes

Human

RUO

wet

2-8C

630 USD