ELISA/assay

Human Hemopexin ELISA

HPX39-K01

1 x 96 well

515 USD

Human Hemopexin ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Hemopexin present in samples reacts with the anti-Hemopexin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-HX antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound HX. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo-genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of HX in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of HX in the test sample. The quantity of HX in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay Kit

Cardiovascular

1 x 96 well

Hemopexin

Hemopexin

2.230 ng/ml

6.25 ng/ml - 200 ng/ml

100 µl

Plasma, Serum, urine

70 minutes

Human

RUO

wet

2-8C

515 USD