ELISA/assay

Hampster (CHO) PLBL2 ELISA

HPB93-K01

1 x 96 well

1510 USD

Hampster (CHO) PLBL2 ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the PLBL2 present in samples reacts with the anti-PLBL2 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-PLBL2 is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. Following another washing step, the complexes are assayed by the addition of a chromo-genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of PLBL2 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of PLBL2 in the test sample. The quantity of PLBL2 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay Kit

Veterinary

1 x 96 well

(CHO)

(CHO)

20 ng/mL - 0.3125 ng/ml

100 µl

Tissue Culture Fluids

170 minutes

Hampster

RUO

wet

2-8C

1510 USD