ELISA/assay

Hydroxyl Radical Antioxidant Capacity (HORAC) Assay

HOR39-K01

1 x 96 well

405 USD

Hydroxyl Radical Antioxidant Capacity (HORAC) Assay

The Hydroxyl Radical Antioxidant Capacity (HORAC) assay is based on the oxidation of fluorescein by hydroxyl radicals via a classic hydrogen atom transfer (HAT) mechanism. Free radicals are generated by hydrogen peroxide (H2O2). The hydroxyl radicals thus generated quench the fluorescence of fluorescein over time. The antioxidants block the hydroxyl radical mediated oxidation of fluorescein until all of the antioxidant activity in the sample is exhausted, after which the H2O2 radicals react with and quench the fluorescence of fluorescein. The area under the fluorescence decay curve (AUC) is used to quantify the total hydroxyl radical antioxidant activity in a sample and is compared to a standard curve obtained using various concentrations of gallic acid. Unlike other antioxidant activity assays, the fluorescent Hydroxyl Radical Antioxidant Capacity (HORAC) assay provides a direct measurement of antioxidant capacity against hydrophilic chain-breaking hydroxyl radicals.

ELISA

Assay Kits

Oxidative Stress

1 x 96 well

HORAC

Hydroxyl Radical Antioxidant Capacity (HORAC) Assay

200 µM

200 - 800 µM

20 µL

Biological Fluids

30 minutes

human

RUO

Wet

2-8C

405 USD