ELISA/assay

Human Lambda ELISA

HLB39-K01

1 x 96 well

610 USD

Human Lambda ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Lambda present in samples reacts with the anti-Lambda antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Lambda antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Lambda. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Lambda in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Lambda in the test sample. The quantity of Lambda in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay Kit

Veterinary

1 x 96 well

Lambda

Lambda

1.329 ng/ml

6.25 ng/ml - 200 ng/ml

100 µl

Serum, plasma, urine

60 minutes

Human

RUO

wet

2-8C

610 USD