ELISA/assay

Human KIM-1 ELISA

HKM39-K01

1 x 96 well

760 USD

Human KIM-1 ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the KIM-1 present in samples reacts with the anti- KIM-1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-KIM-1, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of KIM-1 in the test sample. The quantity of KIM-1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay Kit

Immunology

1 x 96 well

KIM-1

KIM-1

0.054 ng/ml

0.125 ng/ml - 4 ng/ml

100 µl

Serum, plasma, urine

110 minutes

Human

RUO

wet

2-8C

760 USD