ELISA/assay

Horse Haptoglobin ELISA

HHG91-K01

1 x 96 well

630 USD

Horse Haptoglobin ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Haptoglobin present in samples reacts with the anti-Haptoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Hp antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hp. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hp in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hp in the test sample. The quantity of Hp in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay Kit

Veterinary

1 x 96 well

Haptoglobin

Haptoglobin

2.726 ng/ml

18.75 ng/ml - 600 ng/ml

100 µl

Serum, plasma

70 minutes

Horse

RUO

wet

2-8C

630 USD