ELISA/assay

dsRNA ELISA Detection Kit

HG-DS001

8 x 12 wells

1670 USD

dsRNA ELISA Detection Kit

This kit, adopting the principle of double antibody sandwich method and coupling with the streptavidin biotin system, is used for quantitative detection of double stranded RNA (dsRNA) content in samples. The length of dsRNA detected is 60 bp and above, and the dsRNA detected is independent of its nucleic acid sequence. Coat the microtiter plate wells with anti dsRNA antibodies, add samples, incubate and wash, and then incubate with biotinylated detection antibody to form antibody antigen antibody complex, and streptavidin (SA) ho rseradish peroxidase (HRP) conjugate is added after washing again. After washing again, add streptavidin (SA) horseradish peroxidase (HRP) conjugate. After thorough washing, the TMB substrate is added for chromogenic reaction, and TMB is converted to blue under the catalysis of peroxidase and finally to yellow by the termination effect of acid. The shade of color is positively correlated with dsRNA content in the samples. The absorbance (OD value) is measured with a microplate reader at a wavelength of 450 nm, and the dsRNA concentration in the sample is calculated from the standard curve.

ELISA

Assay Kit

Host Cell Protein Detection

8 x 12 wells

dsRNA

dsRNA

The LoD of unmodified, pUTP modified and N1 Me pUTP modified dsRNA is 0.001 pg/μL The LoD of 5 OMe UTP modified dsRNA is 0.01 pg/μL

(5) Dilute unmodified, pUTP modified dsRNA Standard s to 1, 0.5, 0.25, 0.125, 0.0625, 0.0312, 0.0156, and 0 pg/μL with STE buffer. Dilute N1 Me pUTP modified dsRNA Standard s to 2, 1, 0.5, 0.25, 0.125, 0.0625, 0.0312, 0 pg/μL with STE buffer. Dilute 5 OMe UTP modified dsRNA Standard s to 4, 2, 1, 0.5, 0.25, 0.125, 0.0625, 0 pg/μL with STE buffer

100 uL

3 hours

RUO

wet

2-8C

1670 USD