ELISA/assay

Human Ceruloplasmin ELISA

HCP31-K01

1 x 96 well

480 USD

Human Ceruloplasmin ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the CP present in samples reacts with the anti-CP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-CP is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CP in the test sample. The quantity of CP in the sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay kit

cardiovascular

1 x 96 well

Ceruloplasmin

Ceruloplasmin

12.5 - 400 ng/ml

100 µl

Serum, plasma

70 minutes

Human

RUO

wet

2-8C

480 USD