ELISA/assay

Human Alpha 1-Antitrypsin

HAP39-K01

1 x 96 well

465 USD

Human Alpha 1-Antitrypsin

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Alpha 1-Antitrypsin present in samples reacts with the anti-Alpha 1-Antitrypsin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-A1AT antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound A1AT. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A1AT in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A1AT in the test sample. The quantity of A1AT in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay kit

Immunology

1 x 96 well

Alpha 1-Antitrypsin

Alpha 1-Antitrypsin

1.094 ng/ml

7.81 - 500 ng/ml

100 µl

Biological Fluids

100 minutes

Human

RUO

wet

2-8C

465 USD