ELISA/assay

Human Anti-IgE ELISA Assay Kit

HAE31-K01

1 x 96 well

670 USD

Human Anti-IgE ELISA Assay Kit

This Eagle Biosciences Human Anti-IgE Antibody ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human anti-hIgE autoantibodies in serum and plasma samples. The assay utilizes the two-site “bridge” technique with two selected human antibodies. Assay standards, controls and patient samples are directly added to wells of a microplate that is coated with purified human IgE. After the first incubation period, anti-IgE antibodies bind to the human IgE on the wall of microtiter well and unbound proteins in each microtiter well are washed away. A highly purified biotin-labeled human IgE is then added to each microtiter well. After the second incubation period, and a “bridge” of “well coated human IgE = Anti-hIgE antibody = biotin-labeled human IgE” is formed. The unbound protein is removed in the subsequent washing step. HRP-labeled streptavidin is added to the plate wells. For the detection of this immunocomplex, the wells are then incubated with a substrate solution in a timed reaction and subsequently measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the IgE on the wall of the microtiter well is directly proportional to the amount of anti-IgE antibody in the sample. A standard curve is generated by plotting the absorbance versus the respective anti-IgE concentration for each standard on point-to-point or 4 parameter curve fit. The concentration of IgE antibody in test samples is determined directly from this standard curve.

ELISA

Assay Kits

Immunology

1 x 96 well

IgE

IgE ELISA

0.4808 ng/mL

12 - 450 ng/mL

0.2 mL

Serum, Plasma

3 hours

Human

RUO

wet

2-8C

670 USD