GSH/GSSG Cuvette Assay | Eagle BioSciences GSH39-K01 product information

product summary

company name :

Eagle BioSciences

product type :

ELISA/assay

product name :

GSH/GSSG Cuvette Assay

catalog :

GSH39-K01

quantity :

1 x 96 well

price :

560 USD

more info or order :

Eagle BioSciences product webpage

citations: 1

Reference
Mashhoody T, Rastegar K, Zal F. Perindopril may improve the hippocampal reduced glutathione content in rats. Adv Pharm Bull. 2014;4:155-9 pubmed publisher

product information

Eagle Catalog Number :

GSH39-K01

Description :

GSH/GSSG Cuvette Assay

Assay Principle :

The low concentration of GSSG (high GSH/GSSG ratio) in tissues coupled with the need to prevent GSH oxidation during sample preparation, are important considerations for the accurate measurement of GSSG and GSH/GSSG ratios. Guntherberg and Rost (2) first reported the use of N‑ethylmaleimide (NEM) reacting with GSH to form a stable complex, therefore removing the GSH prior to the quantification of GSSG in tissues. Unfortunately, NEM inhibits GR. To overcome this problem, Griffith (3) employed 2‑vinylpyridine (2-VP) to derivatize GSH. Although 2-VP does not significantly inhibit GR, it reacts relatively slowly with GSH and is not very soluble in aqueous solutions.

Product Type :

ELISA

Product Category :

Assay Kits

Product Subcategory :

Oxidative Stress

Size :

1 x 96 well

Biomarker :

Glutathione

Keywords :

GSH, GSSG, Glutathione ratio, gamma glutamylcysteinylglycine,

Sensitivity :

0.1 µM

Standard Range :

0.1 - 3.0 µM

Sample Size :

200 µL

Sample Type :

Biological Fluids

Incubation Time :

20 minutes

Species :

Human

Regulatory Status :

RUO

Shipping :

Wet

Storage :

-20C

LIST Price :

560 USD

more info or order :

Eagle BioSciences product webpage