ELISA/assay

GLP-1 Total ELISA Assay Kit

GP131-K01

1 x 96 well

770 USD

GLP-1 Total ELISA Assay Kit

Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 specific antibody and a horseradish peroxidase (HRP)-conjugated GLP-1 specific antibody is added to each well. After the first incubation period, a “sandwich” immunocomplex of “Streptavidin – Biotin-Antibody – GLP-1(7-36)/(9-36) – HRP-conjugated antibody” is formed and attached to the wall of the plate. The unbound HRP-conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36)/(9-36) on the wall of the microtiter well is directly proportional to the amount of Total GLP-1 in the sample.

ELISA

Assay Kits

Endocrinology

1 x 96 well

GLP-1

GLP-1, glucagon-like-peptide-1

0.6 pmol/L

2 -54 pmol/L

100 µl

Plasma

overnight

Human

RUO

wet

2-8C

770 USD