ELISA/assay

Mouse / Rat Active GLP-1 ELISA

GP121-K01

1 x 96 well

770 USD

Mouse / Rat Active GLP-1 ELISA

The Eagle Biosciences Mouse/Rat Active GLP-1 ELISA Assay Kit is designed, developed and produced for the quantitative measurement of bioactive GLP-1 (7-36) in rodent plasma that usually have limited amount of sample available for analysis. The assay utilizes the two-site “sandwich” technique with two selected GLP-1 (7-36) specific antibodies. Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 (7-36) specific antibody and a horseradish peroxidase (HRP) conjugated GLP-1 (7-36) specific antibody is added to each well. After the first incubation period, a “sandwich” immunocomplex of “Streptavidin – Biotin-Antibody – GLP-1(7-36) – HRP conjugated antibody” is formed and attached to the wall of the plate. The unbound HRP conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36) on the wall of the microtiter well is directly proportional to the amount of GLP-1 (7-36) in the sample.

ELISA

Assay Kits

Endocrinology

1 x 96 well

GLP-1

GLP-1, glucagon-like-peptide-1

0.1 pM

.85 - 146 pM

20 μl

Plasma

24.5 hours

Mouse/Rat

RUO

wet

2-8C

770 USD