ELISA/assay

Total Glutathione Assay Kit

GLU39-K01

1 x 96 well

455 USD

Total Glutathione Assay Kit

The Total Glutathione (tGSH) Microplate Assay kit employs a kinetic enzymatic recycling assay based on the oxidation of GSH by 5,5’-dithiobis(2-nitrobenzoic acid) [DTNB] to measure the total glutathione (tGSH) content of biological samples. The included glutathione standards or treated samples are added to the microtiter plate wells, followed by DTNB and glutathione reductase. Addition of b-NADPH2 to the wells initiates the progressive reduction of DTNB by GSH, causing a color increase that is monitored at 405 nm. The rate of color change, followed over a 5 minute time period, is proportional to the tGSH concentration. Consequently, the concentration of tGSH in unknown samples may be determined by reference to the standard curve. GSH reacts with DTNB to produce a colored ion, which absorbs light at 405 nm, and a mixed disulphide. This disulphide reacts with further quantities of GSH present to liberate another ion and GSSG. GSSG is reduced enzymatically to GSH which then re-enters the cycle (see figure below). Since GSSG represents only a small percentage of total acid-solution free glutathione, the resulting values for tGSH (which encompasses both GSH and GSSG) are expressed in units of GSH equivalents.

ELISA

Assay Kits

Oxidative Stress

1 x 96 well

Glutathione

Glutathione, GSH, TGSH, Total Glutathione,

0.5 µM

0.5 - 20 µM

50 µL

Biological Fluids

20 minutes

Human

RUO

Wet

2-8C/-20C

455 USD