Eagle BioSciences

ELISA/assay

sGAG. Alcian blue-binding assay ELISA

GAG 201

1 x 96 wells

270 USD

GAG 201

sGAG. Alcian blue-binding assay ELISA

The most abundant heteropolysaccharides in the body are the glycosaminoglycans (GAGs). They are located primarily on the surface of cells or in the extracellular matrix. As an example, cartilage is composed to a large extent of GAGs, which are the dominant part of the proteoglycan Aggrecan. GAGs are negatively charged long unbranched polymeric polysaccharides composed of repeating units of disaccharides containing one uronic acid or galactose and one amino sugar, either N-acetylglucosamine or N-acetylgalactosamine. The variation in charge may be very large since each disaccharide is more or less sulphated.

The Eagle Biosciences sGAG Alcian blue binding ELISA Assay is based on the specific interaction between sulphated polymers and the tetravalent cationic dye Alcian blue. The assay is performed at a pH low enough to neutralize all carboxylic and phosphoric acid groups and at an ionic strength large enough to eliminate ionic interactions other than those between Alcian blue and sulphated GAGs (2). The Alcian Blue reagent may be obtained from a number of commercial sources. However, the quality of the reagent and its usability in proteogly can assays differ dramatically from brand to brand. The Alcian Blue reagent in Wieslab’s sGAG assay has been carefully selected and optimised for this particular use. Hyaluronan, a non-sulphated GAG, does not react in this assay. There is no interference from proteins or nucleic acids in this method, in contrast to the DMMB-method or other dye binding methods (2).

ELISA

Assay Kits

Immunology

1 x 96 wells

20 µl

Serum, Plasma, Synovial Fluid

105 min

Human

RUO

wet

4C

270 USD

$230