ELISA/assay

Ficolin-3, Human, ELISA kit, 2 x 96 det.

FIC39-K02

2 x 96 well

1465 USD

Ficolin-3, Human, ELISA kit, 2 x 96 det.

The human Ficolin-3 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured Ficolin-3. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the Ficolin-3 standards (log). The human Ficolin-3 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

ELISA

Assay Kit

Cardiovascular

2 x 96 well

3 ng/ml

3-500 ng/ml

100 ul

Serum, plasma, cell culture supernatants

3.5 hours

Human

RUO

wet

2-8C

1465 USD