Intact FGF-21 ELISA | Eagle BioSciences F2131-K01 product information

product summary

company name :

Eagle BioSciences

product type :

ELISA/assay

product name :

Intact FGF-21 ELISA

catalog :

F2131-K01

quantity :

1 x 96 well

price :

750 USD

more info or order :

Eagle BioSciences product webpage

citations: 1

Reference
Søberg S, Andersen E, Dalsgaard N, Jarlhelt I, Hansen N, Hoffmann N, et al. FGF21, a liver hormone that inhibits alcohol intake in mice, increases in human circulation after acute alcohol ingestion and sustained binge drinking at Oktoberfest. Mol Metab. 2018;11:96-103 pubmed publisher

product information

Eagle Catalog Number :

F2131-K01

Description :

Intact FGF-21 ELISA

Assay Principle :

The Eagle Biosciences Human Intact Fibroblast Growth Factor 21 (FGF-21) ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human intact FGF-21 in serum and EDTA-plasma sample. The assay utilizes the two-site “sandwich” technique with two selected antibodies that bind to different epitopes of human intact FGF-21. One of the antibodies is specifically binds to the N-terminal human FGF-21 (1-7) and the other is specifically to the C-terminal human FGF-21 (175-181). Assay standards, controls and patient samples are added directly to wells of microplate that is coated with an anti-human FGF-21 (1-7) specific antibody. Simultaneously, a horseradish peroxidase conjugated anti-human FGF-21 (175-181) specific antibody is added to each well. After the first incubation period, the antibody on the wall of microtiter well captures human FGF-21 in the sample and unbound proteins in each microtiter well is washed away. A “sandwich” of “anti-FGF-21 antibody --- human intact FGF-21 --- HRP conjugated tracer antibody” is formed. The unbound tracer antibody is removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to human intact FGF-21 on the wall of the microtiter well is directly proportional to the amount of intact FGF-21 in the sample. A standard curve is generated by plotting the absorbance versus the respective human intact FGF-21 concentration for each standard on point-to-point or 4 parameter curve fit. The concentration of human intact FGF-21 in test samples is determined directly from this standard curve.

Product Type :

ELISA

Product Category :

Assay Kits

Product Subcategory :

Endocrinology

Size :

1 x 96 well

Biomarker :

FGF-21

Keywords :

Intact Fibroblast Growth Factor 21, FGF-21

Sensitivity :

1.7 pg/ml

Standard Range :

32.5 - 2000 pg/ml

Sample Size :

50 μl

Sample Type :

Serum, Plasma

Incubation Time :

2.5 hours

Species :

Human

Regulatory Status :

RUO

Shipping :

wet

Storage :

2-8C

LIST Price :

750 USD

more info or order :

Eagle BioSciences product webpage