ENA screen (Extractable Nuclear Antigens) ELISA | Eagle BioSciences ENA31-K01 product information

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product summary

company name :

Eagle BioSciences

product type :

ELISA/assay

product name :

ENA screen (Extractable Nuclear Antigens) ELISA

catalog :

ENA31-K01

quantity :

1 x 96 well

product information

Eagle Catalog Number :

ENA31-K01

Description :

ENA screen (Extractable Nuclear Antigens) ELISA

Assay Background :

Systemic autoimmune diseases such as systemic lupus erythematosus, scleroderma, rheumatoid arthritis, Sjögren’s syndrome, dermatomyositis, mixed connective tissue disease are characterized by the appearance of a variety of autoantibodies directed against components of the cell nucleus.

Assay Principle :

The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP). Within the incubation period of 30 min at RT, excessive conjugate is separated from the solid-phase immune complexes by the following wash step. HRP converts the colorless substrate solution of 3,3’,5,5’-tetramethylbenzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 min at room temperature turning the solution from blue to yellow.

Product Type :

ELISA

Product Category :

Assay Kits

Product Subcategory :

Immunology

Size :

1 x 96 well

Biomarker :

ENA

Keywords :

ENA, Extractable Nuclear Antigens Screen

Sensitivity :

Cut off

Standard Range :

Cut off

Sample Size :

100 ul

Sample Type :

serum, plasma

Incubation Time :

2 hours

Species :

Human

Regulatory Status :

RUO

Shipping :

wet

Storage :

2-8C