ELISA/assay

Human EGF ELISA KIT

EGF31-K01

1 x 96 well

530 USD

Human EGF ELISA KIT

The Eagle Biosciences Human Epidermal Growth Factor (EGF) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Epidermal Growth Factor (EGF) concentrations in cell culture supernates, serum, and plasma. The Eagle Biosciences Human Epidermal Growth Factor (EGF) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

The Eagle Biosciences Human Epidermal Growth Factor (EGF) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for EGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for EGF is added to the wells and binds to the combination of capture antibody- EGF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of EGF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven EGF standard dilutions and EGF sample concentration determined.

ELISA

Assay Kits

Oncology

1 x 96 well

EGF

Epidermal Growth Factor, EGF

15 pg/mL

62.5 - 2000 pg/ml

15 pg/ml

Serum, Plasma, Cell Culture

3.5 hours

Human

RUO

wet

2-8C

530 USD