ELISA/assay

1-Methylhistamine Urine ELISA

EA208/96

1 x 96 well

670 USD

1-Methylhistamine Urine ELISA

The 1-Methylhistamine ELISA Kit contains reagents for the quantitative determination of derivatized 1-methylhistamine in human urine samples. After sample preparation in the preparation plate, derivatization is carried out in the ELISA plate. In this process, 1-methylhistamine is quantitatively converted into N-acyl-1-methylhistamine by the acylation reagent. The 1-methylhistamine ELISA is a competitive enzyme immunoassay. Antigen bound to the solid phase and free antigen in solution compete for a defined number of antibody binding sites. When the system is in equilibrium, the unbound antigen-antibody complex is removed in a washing step and the correspondingly bound complex is detected using a peroxidase conjugate and determined via the conversion of tetramethylbenzidine (TMB). The TMB/POD reaction is stopped and measured at 450 nm. The concentration of the antigen-antibody complex bound to the solid phase is inversely proportional to the concentration of the antigen in the sample.

ELISA

Assay Kits

Immunology

1 x 96 well

Histamine

Histamine, Methylhistamine

0.3

0 - 1000 ng/ml

20 uL

Urine

overnight

Human

RUO

wet

2-8C

670 USD