ELISA/assay

Anti-Pseudomonas aeruginosa ELISA

E15

3 x 96 well

2440 USD

Anti-Pseudomonas aeruginosa ELISA

The Eagle Biosciences anti-Pseudomonas aeruginosa IgG anti-Pseudomonas aeruginosa IgG EIA, E15, is a sandwich enzyme immunoassay. Serum or plasma samples are diluted and added to the wells of a microtiter plate, which have been previously coated with the Pseudomonas aeruginosa Antigenss alkaline protease, elastase or exotoxin A. Specific antibodies in the sample bind to the Antigenss present during an incubation of 2h at 37°C. After washing, the conjugate (anti-human IgG peroxidase-labelled immunoglobulin) is added and incubated again (for 2 h at 37°C). After a final washing step, substrate is added and further incubated for 30 min at room temperature. The reaction is terminated on addition of stop solution accompanied by a change from blue to yellow. The absorbance of the coloured reaction product is measured on a microtiter plate reader. The colour intensity of the reaction corresponds to the concentration of antibodies in the sample.

ELISA

Assay Kits

Immunology

3 x 96 well

Pseudomonas

Anti-Pseudomonas aeruginosa ELISA

0.02 ng/ml

Cutoff

10 ul

Serum, Plasma

4.75 hours

human

RUO

wet

2-8C

2440 USD