ELISA/assay

HAV-Antigens ELISA Kit

E12

1 x 96 well

710 USD

HAV-Antigens ELISA Kit

The Eagle Biosciences specimens are pipetted into wells of a microtiter plate previously coated with antibodies directed against HAV. The HAV Antigens binds to the fixed antibody and after the incubation period of two hours at 37°C the plate is washed thoroughly.  Bound HAV Antigens is identified by conjugate addition (monoclonal anti-HAV, peroxidase conjugated) incubated for another two hours at 37°C. Excess conjugate is removed by washing and the substrate is added. After 30 minutes incubation at room temperature the reaction is terminated by adding stop solution. The blue colour of a positive reaction turns to yellow and is measured in a microplate reader at 450 nm. The intensity of the colour indicates the concentration of bound HAV Antigens.  A positive reaction must be confirmed by neutralising with anti-HAV serum to discriminate false positive reactions which sometimes occurs in stool.

ELISA

Assay Kits

Immunology

1 x 96 well

HAV

HAV, Hepatitis A Virus Antigens

Cutoff

Cutoff

50 ul

Stool, Cell Culture

4.5 hours

human

RUO

wet

2-8C

710 USD