other

functional IGFBP-3 LIA

E04A

1 x 96 well

710 USD

functional IGFBP-3 LIA

The Eagle Biosciences IGFBP-3 LIA Assay Kit utilizes specific and high affinity antibodies for IGFBP-3, the antibodies are immobilized on the microtitre plate. The ligand, biotinylated IGF-I is pre-dispensed in excess into the needed wells. The sample is diluted outside within a special dilution buffer (Sample Buffer PP); all naturally bound IGFs are thereby released from their binding proteins. By adding an aliquot of such diluted sample with free IGFBP-3 to the ligand containing wells, the biotinylated IGF-I occupies all existing specific binding sites of the IGFBP-3. All IGFBP-3 molecules are bound afterwards to the microtitre plate, however by using a Strepavidin-Peroxydase-(POD)-Conjugate only the complexes of IGFBP-3/biotinylated IGF-I are involved in signal generation (in the closing POD-substrate reaction), thus, only functional IGFBP-3 is being quantified.

LIA

Assay Kits

Endocrinology

1 x 96 well

IGFBP-3

IGFBP-3, Insulin Growth Factor Binding Protein

0.4 ng/mL

0.4 - 30 ng/mL

10 ul

Serum, Plasma

3.5 hours

Human

RUO

wet

2-8C

710 USD