ELISA/assay

Anti-Deamidated Gliadin Peptides (DGP) IgA ELISA

DKO107

1 x 96 well

515 USD

Anti-Deamidated Gliadin Peptides (DGP) IgA ELISA

Anti Deamidated Gliadin Peptide (DPG) IgA test is based on the binding of present antibodies in calibrators, controls or prediluted patient samples on the syntetic deamidated Gliadin peptides (DGP) coated on the inner surface of the wells. After a 30 minutes incubation the microplate is washed with wash buffer for removing non-reactive serum components. An anti-human-IgA horseradish peroxidase conjugate solution recognizes IgA class antibodies bound to the immobilized antigens. After a 30 minutes incubation any excess enzyme conjugate, which is not specifically bound is washed away with wash buffer. A chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation the color development is stopped by adding the stop solution. The solutions color changes into yellow. The amount of color is directly proportional to the concentration of IgA antibodies present in the original sample.

ELISA

Assay Kit

Gastroenterology

1 x 96 well

Anti-Deamidated Gliadin Peptide, DGP

Anti-Deamidated Gliadin Peptide, DGP

0.74 AU/mL

15 - 240 AU/mL

10 uL

Serum, Plasma

1 hour 15 minutes

Human

RUO

wet

2-8C

515 USD