ELISA/assay

Anti CP IgG ELISA

DCM149

1 x 96 well

505 USD

Anti CP IgG ELISA

The Anti CP IgG ELISA Assay Kit is based on the binding of antibodies present in calibrators, controls or prediluted patient samples to the syntetic Citrullinated Peptides (CP) coated on the inner surface of the wells. After a 30 minutes incubation the microplate is washed with a wash buffer to remove the non-reactive serum components.  Then an anti-human-IgG horseradish peroxidase conjugated solution recognizes the IgG class antibodies bound to the immobilized Antigenss. After a 30 minutes incubation, any excess of enzyme conjugate not specifically bound is washed away with the wash buffer.  Finally a chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation the color development is stopped by adding the stop solution. The solutions color changes into yellow. The amount of color is directly proportional to the concentration of IgG antibodies present in the original sample.

ELISA

Assay Kits

Immunology

1 x 96 well

CP

CP, citrullinated peptides, rheumatoid arthritis

5 AU/ml

5 - 80 AU/ml

10 ul

Serum, Plasma

1.5 hours

Human

RUO

wet

2-8C

505 USD