ELISA/assay

Anti-Phospholipid Screen ELISA Assay Kit

DCM114

1 x 96 wells

565 USD

Anti-Phospholipid Screen ELISA Assay Kit

Anti Phospholipid Screen test is based on the binding of antibodies in human serum directed against the antigenic complex between anionic phospholipids (Cardiolipin, Phosphatidyl serine, Phosphatidyl inositol, Phosphatidic acid, Phosphatidyl choline, Lyso-phosphatidyl choline, Phosphatidyl ethanolamine) and β2-Glycoprotein; these complexes are coated on the microplate. Any antibody of IgG class or IgM class in calibrators, controls or prediluted patient samples binds to its respective antigen. After 60 minutes incubation, the microplate is washed with wash buffer for removing non-reactive serum components. An anti-human IgG conjugate solution (Conjugate IgG, reactive 4) or an anti-human IgM conjugate solution (Conjugate IgM, reactive 5) recognize IgG class or IgM class antibodies, respectively, bound to the immobilized antigens. After a 30 minutes incubation any excess enzyme conjugate which is not specifically bound is washed away with the wash buffer. A chromogenic substrate solution containing TMB is then dispensed into the wells. After a 15 minutes incubation the color development is stopped by adding the stop solution. The solutions color change into yellow. The amount of color is directly proportional to the concentration of IgG or IgM antibodies present in the original sample. The concentration of IgG or IgM antibodies in the original sample is calculated through a calibration curve.

ELISA

Assay Kit

Immunology

1 x 96 wells

0.03 AU/mL for IgG and 0.16 AU/mL for IgM

0 - 80 AU/mL

10 uL

Serum, Plasma

1 hour 45 minutes

Human

RUO

wet

2-8C

565 USD