ELISA/assay

ANTI CARDIOLIPIN IgM ELISA

DCM112

1 x 96 well

445 USD

ANTI CARDIOLIPIN IgM ELISA

Anti Cardiolipin IgM ELISA Assay Kit is based on the binding of antibodies directed against the Antigensic complex between Cardiolipina and β2-Glycoprotein on human serum or plasma; this complex is coated on the microplate. In the first step, the antibodies present in calibrators, controls or prediluted samples bind to this Antigens. After a 60 minutes incubation the microplate is washed with a wash buffer to remove the non-reactive serum or plasma components. Then an anti-human IgM horseradish peroxidase conjugated solution recognizes the IgM class antibodies bound to the immobilized Antigenss. After a 60 minutes of incubation, any excess enzyme conjugate, which is not specifically bound, is washed away with the wash buffer. Finally, achromogenic substrate solution containing TMB is dispensed into the wells. After a 15 minute incubation the color development is stopped by adding the stop solution. The solution turns yellow at this point. The level of color is directly proportional to the concentration of IgM antibodies present in the original sample.The concentration of IgM antibodies in the sample is calculated through a calibration curve.

ELISA

Assay Kits

Immunology

1 x 96 well

ANTI_CARDIOLIPIN_IGM

ANTI CARDIOLIPIN IGM

0.12 AU/ml

5 - 80 AU/ml

10 ul

Serum, Plasma

2 hours

Human

RUO

wet

2-8C

445 USD